Study of Sickle cell disease using magnetic resonance: potential medical applications

Authors

Keywords:

Magnetic Resonance, Sickle cell disease, hemoglobin-membrane interaction, water exchange through membrane, dynamic viscosity of blood plasma

Abstract

Introduction: Sickle cell disease is a hematological illness needed of new experimental methods to improve the patient clinical evaluation and the test of new therapeutic options. Proton magnetic relaxation has been widely used to study this disease with important, but controversial results and limited in their usefulness.

Objective: To provide new knowledge that explains the protonic magnetic relaxation during the polymerization of hemoglobin.

Methods: They were used different magnetic resonance methods to determine the proton magnetic relaxation times, the hemoglobin-membrane interaction, the water exchange time through membrane and the dynamic viscosity of blood plasma in patients with Sickle cell disease. The experiments were carried out at 36°C, intraerythrocytic cellular concentration and pH of 7.4.

Results: Both relaxation times showed similar behaviors during hemoglobin S polymerization and the dispersion of the longitudinal relaxation in hemoglobin solutions was fitted to one three sites model for water exchange with two populations of internal water and labile protons exchanging with the solvent. The hemoglobin rotational correlation time, the micro viscosity around this protein and the hemoglobin-membrane interaction increased, and the water exchange time through membrane decreased, during macromolecular agglutination. The dynamic viscosity of blood plasma increased in patients with this disease.

Conclusions: The reduction of mobility of the two populations of internal water identified allowed to explain the proton magnetic relaxation behavior during hemoglobin S polymerization.  The delay time, as well as the initial and final values of the transversal proton magnetic relaxation time, were useful for the clinical evaluation of the patients and for the tests of new therapeutic options. The water exchange time through membrane and the dynamic viscosity of blood plasma, determined using proton magnetic relaxation, showed similar potentialities.

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Published

2025-04-01

How to Cite

Lores Guevara, M. A., Cabal Mirabal, C. A., Fernández García, A. A., Mengana Torres, Y., García Naranjo, J. C., Rodríguez Reyes, I., … Alonso Gelis, Y. (2025). Study of Sickle cell disease using magnetic resonance: potential medical applications. Anales De La Academia De Ciencias De Cuba, 15(1), 2896. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/2896

Issue

Vol. 15 No. 1: January-March 2025 (Issue in progress)

Section

Biomedical sciences

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