Pathophysiological mechanisms that trigger neuronal death in drug-resistant temporal lobe epilepsy

Authors

Keywords:

drug-resistant temporal lobe epilepsy, pathophysiological mechanisms, inflammation, oxidative stress, neuronal death

Abstract

Introduction. In relation to drug-resistant temporal lobe epilepsy (DRTLE), the interrelation between inflammation, oxidative stress, and neuronal death constitutes a topic increasingly researched into. Objectives: To expand the knowledge of the molecular mechanisms that participate in the pathophysiology of DRTLE by correlating clinical variables with immunological ones, oxidative stress and neuronal death.
Methods. Immunocytochemistry techniques were used to measure the markers CD3, CD4, CD8, CD19, CD25, HLA-DR; Nephelometry was used for IgG, IgM, IgA, and Albumin; immunoenzymatic tests for IL-1β, IL-6, TNF-α, and nitric oxide. By means of western blot: NFκB, JNK, 4-hydroxy-2-nonenal, 3-nitrotyrosine; by means of spectrophotometry: malonildialdehyde, advanced glycation products, advanced protein oxidation products, uric acid, superoxide dismutase, glutathione, and vitamin C; by means of immunohistochemistry: Annexin V, TUNEL, synaptophysin, caspase 3, p53, and bcl-2; by means of HPLC: glutamate and by means of autorradiography: 5-HT, 5-HT1A, 5-HT7.
Results. It is shown that seizures are the pathogenic substrate of immunological, inflammatory and oxidative stress disorders in DRTLE. The clinical variables are related to the molecular and protein studies conducted and all together show the neuronal death in the DRTLE.

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Author Biography

Lourdes Lorigados Pedre, Centro Internacional de Restauración Neurológica

Jefe Dpto Inmunoquimica

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Published

2020-10-10

How to Cite

Lorigados Pedre, L., Morales Chacón, L. M., Pavón Fuentes, N., Serrano Sánchez, T., Robinson Agramonte, M. de los A., González Fraguela, M. E., … Gallardo Montoya, J. M. (2020). Pathophysiological mechanisms that trigger neuronal death in drug-resistant temporal lobe epilepsy. Anales De La Academia De Ciencias De Cuba, 10(3), e798. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/798

Issue

Vol. 10 No. 3 (2020): septiembre-diciembre

Section

Biomedical sciences

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