Unilateral neurotoxic lesion of the pedunculopontine nucleus: a good approach to the pre-symptomatic experimental model of Parkinson disease in rats

Authors

  • Lisette Blanco Lezcano Centro Internacional de Restauración Neurológica (CIREN)
  • Esteban Alberti Amador CIREN
  • María Elena González Fraguela CIREN
  • Mei-Li Díaz Hung CIREN
  • Bárbara Estupiñán Díaz CIREN
  • Teresa Serrano Sánchez CIREN
  • Yamilé Vega Hurtado CIREN
  • Isabel Fernández Jiménez CIREN

Keywords:

pedunculopontine nucleus, substantia nigra pars compacta, neurodegeneration, oxidative stress

Abstract

Introduction. The pedunculopontine nucleus (NPP) plays an essential role in motor control. Literature on the topic suggests that PPN participates in the onset and progression of Parkinson's disease (PD) and that its early degeneration precedes the death of nigral dopaminergic neurons. Objectives: to determine the effect of neurotoxic injury of PPN on motor function, nigrostriatal oxidative homeostasis and gene expression of proteins responsible for dopaminergic homeostasis.
Methods. Motor performance was evaluated by beam, cylinder and footprint tests. Biochemical studies focused on catalase enzymatic activity (CAT), cholinesterase (AChE AE) and concentrations of malondialdehyde (MDA) and glutathione (GSH). The molecular studies included the expression of tyrosine hydroxylase (TH) mRNA; vesicular dopamine transporter (VMAT), brain-derived neurotrophic factor (BDNF) and dopamine transporter (DAT).
Results. Behavioral studies revealed slight coordination disorders and subtle changes in the walking pattern of rats with pontine injury. Biochemical studies showed an early increase in CAT activity as well as concentrations of MDA and GSH. Molecular studies demonstrated a transient increase in nigral expression of BDNF and TH together with a marked decrease in gene expression of VMAT and DAT.

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Author Biography

Lisette Blanco Lezcano, Centro Internacional de Restauración Neurológica (CIREN)

Jefa del Departamento de Neurofisiología Experimental del Centro de Investigaciones del Centro Internacional de Restauración Neurológica

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Published

2020-10-11

How to Cite

Blanco Lezcano, L., Alberti Amador, E., González Fraguela, M. E., Díaz Hung, M.-L., Estupiñán Díaz, B., Serrano Sánchez, T., … Fernández Jiménez, I. (2020). Unilateral neurotoxic lesion of the pedunculopontine nucleus: a good approach to the pre-symptomatic experimental model of Parkinson disease in rats. Anales De La Academia De Ciencias De Cuba, 10(3), e800. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/800

Issue

Vol. 10 No. 3 (2020): septiembre-diciembre

Section

Biomedical sciences

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