Potentially active polyphenols identified in plant extracts of the Cuban flora
Keywords:
cuban flora, polyphenols, sesquiterpene lactone, antioxidant activity, antiviral activityAbstract
Introduction: Maytenus buxifolia subsp. cajalbanica Borhidi & O. Muñiz, Ageratina havanensis (Kunth) R. M. King & H. Rob, mucuna (Mucuna pruriens [L] DC. vc. utilis, Stizolobium niveum y Stizolobium aterrimum), Coccoloba cowellii Britton plants and the brown algae Sargasssum fluitams present potential for their use in medicinal chemistry and agriculture. The objective of this work was to study their extracts using phytochemical techniques of isolation, characterization, identification and quantification of their secondary metabolites, as well as by evaluating their biological activity.
Methods: They were isolated and characterized the flavonoids and sesquiterpene lactone reported by chromatographic and spectroscopic techniques, respectively. They were identified and quantified metabolites by tandem mass spectrometry in the direct injection mode of the sample and coupled to a high-resolution liquid ultra-chromatograph. It was determined antioxidant activity in vitro by the trapping capacity of the DPPH radical and the reduction of ferric iron in the FRAP assay and in vivo using rats as a laboratory animal in a carbon tetrachloride-induced intoxication model. It was evaluated in vitro antiviral activity against Dengue virus 2 and human enteroviruses: coxsackievirus A16, echovirus 9 and coxsackievirus A24.
Results: They were identified Polyphenolic compounds of the family of proanthocyanidins and flavonoids in most of the plants studied. They were isolated one sesquiterpene lactone novel to science and seven flavonoids. They were quantified different types of metabolites and especially the major flavonoids of Ageratina havanensis by tandem mass spectrometry coupled to a high-resolution liquid ultrachromatograph. They were found antioxidant and antiviral properties and effects on P-glycoprotein function in the plants studied. Conclusions: They were identified polyphenol compounds in most of the plants studied, which justifies the biological properties found in their extracts. Furthermore, it was isolated and identified a sesquiterpene lactone for the first time. These results constitute an important contribution to the knowledge of our flora and its potential in bioactive natural products.Downloads
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