Obtaining models of dementia in mouse strains and their application in the development of new drugs

Authors

Keywords:

behavioral tests, cognitive function, Alzheimer's dementia

Abstract

Introduction: Dementia is a clinical syndrome that involves the decline of various cognitive functions.

Objective: To obtain animal models of dementia with mice strains produced in Centro para la production de animales de laboratorio and to demonstrate its usefulness in the development of a formulation for its treatment.

Methods: Young animals of the strains OF1cenp and C57BL/6cenp were evaluated with three compounds that were administered intracerebroventricularly to obtain an Alzheimer-type dementia model. In addition, it was carried out the neurobehavioral, hematological, biochemical and histological characterization of the strains BALB/ccenp, OF1cenp and C57/BL/6cenp of aged mice as a model of dementia. These models were evaluated for the development of the new biologic drug Biocen-128.

Results: OF1cenp strain responded better than the C57BL/6cenp to the Alzheimer's model by administration of streptozotocin and in this model, the anti-neuroinflammatory effect of the BioCen-128 formulation was demonstrated. In the three strains of aged mice there was observed deterioration in cognitive function, in BALB/ccenp and OF1cenp mice an increase in oxidative damage was found; and in BALB/ccenp and C57BL/6cenp there was a decrease in thymus weight. In addition, brain weight decreased in all three strains studied. An increase in nuclear polymorphs and a decrease in lymphocytes were observed in C57BL/6cenp mice. In the BALB/ccenp and C57BL/6cenp strains, the positive effect of BioCen-128 was demonstrated with respect to behavioral, hematological and biochemical parameters.

Conclusions: Male mice of the strain OF1cenp (15-16 months) and BALB/ccenp (12-13 months) and females of the strain C57BL/6cenp (15-16 months), can be used as a model of dementia. The dementia models developed were validated with the application of BioCen-128 since characteristic markers of Alzheimer's dementia were modified.

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Published

2024-07-01

How to Cite

García Hernández, Y., Esquivel Crespo, N., Rodríguez Martínez, C., Morejón Calderón, A., Vargas Caballero, Y., Menéndez Soto del Valle, R., & García Jiménez, T. E. (2024). Obtaining models of dementia in mouse strains and their application in the development of new drugs. Anales De La Academia De Ciencias De Cuba, 14(2), e1593. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/1593

Issue

Vol. 14 No. 2: arpil-june 2024 (Issue in Progress)

Section

Biomedical sciences

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