Sticholysin II encapsulated into liposomes enhances an antigen-specific cytotoxic T lymphocytes response

Authors

  • Maria Eliana Lanio Ruiz universidad de La Habana, MES
  • Rady Judith Laborde Quintana
  • Yoelys Cruz Leal
  • Luis Enrique Fernández Molina
  • Oraly Sánchez Ferrás
  • Anaixis del Valle
  • Isbel Lopetegui González
  • María C. Luzardo Lorenzo
  • Carlos Álvarez Valcárcel
  • Circe Mesa

Keywords:

pore-forming proteins, liposomes, antigen presenting cells, antigen cross-presentation, cytotoxic T lymphocytes

Abstract

Introduction: Vaccine strategies to enhance CTLs response are still a challenge since it is necessary to favor the exogenous antigens (Ag) cross-presentation by antigen presenting cells (APCs). For this purpose, liposomes encapsulating bacteria pore-forming proteins (PFPs) have been used. Objective. To explore the ability of liposomes co-encapsulating the Ag ovalbumin (OVA) and sticholysin II (StII), a PFP produced by Stichodactyla helianthus, to induce a CTL response, functional in a tumor model.
Methods: Liposomes co-encapsulating OVA and StII were prepared by dehydration-rehydration (Lp/OVA/StII). The response of CTLs was evaluated, as well as the antitumor activity in a preventive setting. The ability of StII to stimulate maturation of dendritic cells (DCs) in vitro was also explored.
Results: Lp/OVA/StII enhanced the OVA-specific CTL response and antitumor activity in mice challenged with the E.G7-OVA tumor. StII induced the maturation of DCs in vitro, which was dependent on the Toll-like receptor 4 (TLR4), with a contribution to the response of CTLs induced by the liposomal formulation in vivo. Lp/OVA/StII favored in vitro the cross-presentation of OVA and the activation of CD8+ T cells by macrophages, but not by DCs. These results are the first evidence that StII encapsulated into liposomes functions as a vaccine platform to induce a robust CTL response, with a contribution that goes beyond its ability to form pores in membranes.

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Author Biography

Maria Eliana Lanio Ruiz, universidad de La Habana, MES

Prof Consultante, Dra en Ciencias Biologicas, Centro de Estudios de Proteínas (CEP), Facultad de Biología, Universidad de La Habana

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Published

2021-01-02

How to Cite

Lanio Ruiz, M. E., Laborde Quintana, R. J., Cruz Leal, Y., Fernández Molina, L. E., Sánchez Ferrás, O., del Valle, A., … Mesa, C. (2021). Sticholysin II encapsulated into liposomes enhances an antigen-specific cytotoxic T lymphocytes response. Anales De La Academia De Ciencias De Cuba, 11(1), e821. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/821

Issue

Vol. 11 No. 1 (2021): enero-abril

Section

Biomedical sciences

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