Obtention of fungal laccases for the improvement of the fibrous sources for animal feed
Keywords:
animal production, cell wall, degradation, Fungi, enzymesAbstract
Introduction: The use of lignolytic enzymes in the field of animal production is an issue that has not yet been developed.
Objective: To obtain and evaluate fungal laccases for use in animal production.
Methods: It was selected the strain with the highest lignocellulolytic potential from several isolates of fungi. It was evaluated their laccase production with and without biological induction. Laccases were purified by triphasic partitioning and characterized in terms of pH and temperature of maximum activity, stability and heat resistance. They were evaluated native and induced laccases as a pretreatment method for raw wheat straw and sugarcane bagasse. It was monitored fiber modification by Fourier Transformed Infrared Spectroscopy. It was analyzed the effect of enzymatic pretreatment in improving the nutritional quality and digestibility in vitro and in vivo (rabbit and broiler) of sugarcane bagasse.
Results: The Curvularia kusanoi L7 strain was selected as the one with the highest enzyme production. They were purified both native and induced laccases with yields greater than 100% and with purification factors of 20. The induced enzymes were different from the native in terms of activity, pH and heat resistance. Both types were able to modify the structure of lignin, improve the nutritional quality and the in vitro and in vivo digestibility of the sugarcane bagasse.
Conclusions: Fungal laccases constitute a new variant of pretreatment of fibrous sources for animal production, and it is also interesting to agricultural and biotechnological field.Downloads
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