Alternatives for the propagation of Bambusa vulgaris Schard. ex Wendl

Authors

  • Yudith Yanet García Ramírez Instituto de Biotecnología de las Plantas https://orcid.org/0000-0002-9873-4285
  • Marisol Freire Seijo
  • Mallelyn González González
  • Yelenys Alvarado Capó
  • Mayra Acosta Suárez
  • Ortelio Hurtado Ribalta
  • Ambrosio Amado Pérez Cabrera
  • Raúl Barbón Rodríguez
  • Miladys León Quintana
  • Mariana la O Cárdenas
  • Leonardo Ribero Quintana
  • Sinesio Torres García
  • Ángel Mollineda Trujillo
  • Fernando Martirena Hernández
  • Pedro Seijo Pérez
  • Berto Arteaga Arbolaes
  • Armando Solano Cabrera

Keywords:

Bamboo, macropropagation, in vitro propagation

Abstract

Introduction. The high rate of visible microbial contaminants in the establishment phase, as well as a low rate of multiplication, rooting and survival ex vitro, constitute the main problems during in vitro propagation in Bambusa vulgaris Schard. exWendl. The present work was carried out with the objective of proposing new alternatives for the propagation of Bambusa vulgarisSchard. exWendl combining traditional techniques.
Methods. The influence of climatic conditions on the in vitro establishment of stem segments was determined. In addition, the effect of the culture conditions on the multiplied shoots in static liquid culture medium and temporary immersion systems was studied, as well as their response during the acclimatization phase.
Results. Microbial contaminants with a high frequency of appearance associated with plants in cultivation and in vitro were identified for the first time. During the in vitro establishment of the axillary buds, 99 % sprouting and 98 % explants free of visible microbial contaminants were achieved between the months of January-April and November-December. It was shown that shoots multiplied between the sixth and eighth subculture with 12 μM 6-BAP can be used for multiplication in Temporary Immersion Systems. It was possible to increase the morpho-physiological and biochemical quality of the shoots grown in the SITs with the lowest concentration of 6-BAP (6,0μM) and an immersion time of 2 min, aspects little addressed in the bamboo species. The first studies in the species on the effect of sucrose on the morpho-physiological, anatomical and biochemical response of shoots in SITs during the in vitro- ex vitro transition were described. The plants brought to the grow house were able to emit between 4 and 7 new shoots with high survival rates during the extraction of suckers.

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Author Biography

Yudith Yanet García Ramírez, Instituto de Biotecnología de las Plantas

MSc. Yudith García Ramírez. Instituto de Biotecnología de las Plantas, UCLV.

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Published

2020-09-12

How to Cite

García Ramírez, Y. Y., Freire Seijo, M., González González, M., Alvarado Capó, Y., Acosta Suárez, M., Hurtado Ribalta, O., … Solano Cabrera, A. (2020). Alternatives for the propagation of Bambusa vulgaris Schard. ex Wendl. Anales De La Academia De Ciencias De Cuba, 10(3), e823. Retrieved from https://revistaccuba.sld.cu/index.php/revacc/article/view/823

Issue

Vol. 10 No. 3 (2020): septiembre-diciembre

Section

Agricultural and Fishery Sciences

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